Journal: Marine Drugs

Article Title: Microbial Population Changes in Decaying Ascophyllum nodosum Result in Macroalgal-Polysaccharide-Degrading Bacteria with Potential Applicability in Enzyme-Assisted Extraction Technologies

doi: 10.3390/md17040200

Figure Lengend Snippet: Observed OTUs and species richness and diversity estimates of Ascophyllum -associated metagenomic communities obtained using MiSeq sequencing of the 16S rRNA gene from the intact seaweed (T 0 ) and each of decaying Ascophyllum nodosum samples collected at three phases of the decay period (week 2, week 4 and week 6) at 18 °C (2_18, 4_18, 6_18), 25 °C (2_25, 4_25, 6_25), and at 30 °C (2_30, 4_30, 6_30).

Article Snippet: Sanger sequencing was performed on the amplified PCR products by GATC Biotech, (Konstanz, Germany) and Macrogen (Amsterdam, The Netherlands).

Techniques: Sequencing

Journal: Marine Drugs

Article Title: Microbial Population Changes in Decaying Ascophyllum nodosum Result in Macroalgal-Polysaccharide-Degrading Bacteria with Potential Applicability in Enzyme-Assisted Extraction Technologies

doi: 10.3390/md17040200

Figure Lengend Snippet: Relative abundances of bacterial phyla associated with intact (T 0 ) and decaying Ascophyllum nodosum at 2, 4, and 6 weeks of decay at 18 °C (2_18, 4_18, 6_18); 2, 4, and 6 weeks of decay at 25 °C (2_25, 4_25, 6_25), and 2, 4, and 6 weeks of decay at 30 °C (2_30, 4_30, 6_30) obtained from metagenomic 16S rRNA gene sequencing. The relative distribution of phyla in each group is represented as a percentage.

Article Snippet: Sanger sequencing was performed on the amplified PCR products by GATC Biotech, (Konstanz, Germany) and Macrogen (Amsterdam, The Netherlands).

Techniques: Sequencing

Journal: Anais Brasileiros de Dermatologia

Article Title: No detection of c-kit gene mutations in exons 9, 11, 13 and 17 and low CD117 expression in plaque-stage mycosis fungoides *

doi: 10.1590/abd1806-4841.20187930

Figure Lengend Snippet: Material and methods

Article Snippet: DNA standard sequencing by Sanger method with Applied Biosystems 3730xl DNA Analyzer (Macrogen ® , South Korea)..

Techniques: DNA Extraction, Purification, Amplification, Sequencing, Expressing, Positive Control

Journal: The Journal of General Virology

Article Title: Precision engineering of human cytomegalovirus without BAC constraints: a Sendai virus-delivered CRISPR/Cas9 approach

doi: 10.1099/jgv.0.002126

Figure Lengend Snippet: Dual editing of the HCMV genome with two SeV-Cas9 viruses. ( a ) A schematic of the HCMV pentamer gene block and the relative location of the UL128 g1 and UL130 g1 cut sites. The bottom image depicts the 663 bp deletion that is predicted to occur if SeV-Cas9 UL128g1 and SeV-Cas9 UL130 were employed in the same editing experiment. ( b ) Long-read ONT amplicon sequencing of HCMV edited by one (UL130g1, top panel) or two (UL128g1+UL130 g1, bottom panel) SeV-Cas9 targeting the PC. The read distributions are shown. Data from these reads are plotted in ( c ). Viral DNA from HCMV virions edited either by one PC targeting SeV-Cas9 or by two guides was extracted and analysed by ONT sequencing. The percentages of total reads containing the predicted perfect deletion are mapped for each sample. This experiment was repeated in two biological replicates. ( d ) HCMV virus edited by the single PC-targeting SeV-Cas9 or by the combination was titrated on MRC5 fibroblasts to determine the infectious unit per millilitre, and viral DNA was extracted and genome copy number per millilitre was calculated by quantitative PCR (qPCR). The genome copy versus infectious units was plotted from three independent replicates, and the differences were not statistically significant, as determined by multiple unpaired t-tests. ( e ) UL128g1, UL130g1 or combination edited HCMV virus was titrated on MRC5s and ARPE-19s, and the per cent infectivity was calculated. Statistics were calculated using a one-way ANOVA test with P <0.0001.

Article Snippet: PCR products were purified and sent for sequencing analysis (Macrogen), and Sanger sequencing files were analysed for CRISPR editing efficiency with ICE (Synthego).

Techniques: Blocking Assay, Amplification, Sequencing, Virus, Real-time Polymerase Chain Reaction, Infection